Here we offer to the lipidomic community the LipidQuant 1.0 tool for automated data processing in the lipidomic quantitation based on lipid class separation coupled with high-resolution mass spectrometry (MS), e.g., hydrophilic interaction liquid chromatography (HILIC), ultrahigh-performance supercritical fluid chromatography (UHPSFC), or normal-phase (LC) liquid chromatography. The advantage of lipid class separation workflows for the lipidomic quantitation is the coionization of lipid class internal standards with analytes from the same class, which guarantee the robust quantation in line with MS recommendations. The individual steps in the LipidQuant 1.0 workflow are explained in the paper published in Bioinformatics on September 8, 2021.
The LipidQuant 1.0 processing tool and the preprint of manuscript are available on:
Wolrab, Denise, Cifkova, Eva, Can, Pavel, Lisa, Miroslav, Peterka, Ondrej, Chocholouskova, Michaela, Jirasko, Robert, & Holcapek, Michal (2021). LipidQuant 1.0: automated data processing in lipid class separation - mass spectrometry quantitative workflows. In Bioinformatics (LipidQuant 1.0). Zenodo. https://doi.org/10.5281/zenodo.5151201
ReadMe file with instructions how to use LipidQuant 1.0 (updated on July 31, 2021)